| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Grimley, Taylor O (10)
|
MARAD |
43.75 |
42.72 |
-1.03 |
| 2 |
Lin, Simone (10)
|
MAPAAC |
44.21 |
42.75 |
-1.46 |
| 3 |
Sergent, Brooke D (10)
|
MALVA |
48.04 |
42.87 |
-5.17 |
| 4 |
Tewksbury, Madison O (10)
|
MAPAAC |
46.34 |
43.80 |
-2.54 |
| 5 |
Neilly, Hannah F (10)
|
MARAD |
52.41 |
44.20 |
-8.21 |
| 6 |
Settle, Ashley (10)
|
MAPAAC |
52.12 |
45.77 |
-6.35 |
| 7 |
O'Neill, Eleanor G (10)
|
MARAD |
46.33 |
46.28 |
-0.05 |
| 8 |
Herman, Alyvia L (9)
|
MAPAAC |
51.70 |
46.85 |
-4.85 |
| 9 |
Dahlgren, Brenna (9)
|
MAUN |
50.65 |
46.97 |
-3.68 |
| 10 |
Piacenti, Lily (10)
|
MAPFY |
53.88 |
47.30 |
-6.58 |
| 11 |
Karnhaukhova, Anastasiya (10)
|
MAUN |
|
47.31 |
- |
| 12 |
Mlodzienski, Sophia (10)
|
MARAD |
52.09 |
50.51 |
-1.58 |
| 13 |
Otero-Rehrig, Hazel C (9)
|
MALVA |
1:06.26 |
51.28 |
-14.98 |
| 14 |
Ritter, Kassie M (9)
|
MAEAST |
|
51.66 |
- |
| 15 |
Nagy, Samantha K (9)
|
MAEAST |
57.14 |
52.37 |
-4.77 |
| 16 |
Gandhi, Reva (10)
|
MAPAAC |
56.65 |
52.74 |
-3.91 |
| 17 |
Connell, Samantha R (9)
|
MALVA |
1:06.10 |
59.41 |
-6.69 |
| 18 |
Sandt, Meghan M (9)
|
MAEAST |
1:11.87 |
1:05.74 |
-6.13 |
| 19 |
Chukoskie, Emily P (9)
|
MAPAAC |
1:07.54 |
1:06.21 |
-1.33 |
|
Barroquiero, Addisen C (9)
|
MAEAST |
|
DQ |
- |
|
Bevenour, Holly E (10)
|
MARAD |
52.62 |
NS |
- |
|
Hahn, Lily (10)
|
MARAD |
48.82 |
NS |
- |